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. 2017 Aug 24;8(56):95316–95331. doi: 10.18632/oncotarget.20517

Figure 6. Metabolic activity comparison of 2D vs 3D cell cultures with resazurin test.

Figure 6

The resazurin is reduced into resorufin by metabolically active cells. The amount of resorufin formed thus reflects the metabolic activity of the cells. Corrected OD of resorufin (λ570- λ620 nm) was measured after 6h of incubation with resazurin in PBS (60 μM) in the presence of 1000 cells per well at seeding for both cell lines and both culture conditions. Measures were made for both cell lines (A) SUM1315 and (B) MDA-MB-231 and all conditioning regimens after one day of culture. Graphs represent at least triplicate biological repeats and are displayed as mean ± SEM, ***** = p<0.00001.