Figure 3. EV20-Sap but not naked EV20 or free Saporin demonstrates cell killing activity in melanoma cells.
(A) SK-MEL 2 cells growing in complete medium were treated with EV20-Sap, EV20 and free Saporin, as indicated. After 5 days cell viability was measured by MTT staining. Mean +/- SD (n=3). (B) SK-MEL 24 cells were seeded in 24-wells plate at a density of 5×103 and allowed to attach and propagate overnight before the treatments with EV20-Sap (1.9 nM). The ADC was removed (wash out) at different time points and complete medium replaced until the end of experiment. Cells treated continuously for 120 hrs were used as control. Cell viability was assessed by MTT staining. OD: optical density. Mean +/- SD (n=3).