Table 1. All samples of total RNA quality test results.

Sample ID	Sample name	K5500			AGE	Agilent 2200		Detection conclusion
		OD260/280	OD260/230	Total amount (ug)		RIN	28S/18S
1	T-PBS	1.87	2.00	15.99	pass	10.0	3.2	A
2	T-PBS-6.21	1.85	1.91	21.04	pass	10.0	3.0	A
3	T-PBS-6.23	1.90	2.04	28.88	pass	10.0	3.4	A
4	T-20	1.79	2.05	16.38	pass	10.0	3.4	A
5	T-20-6.21	1.90	1.64	22.50	pass	10.0	2.3	A
6	T-20-6.23	1.84	1.98	23.53	pass	10.0	2.8	A

T-PBS, T-PBS-6.21, T-PBS-6.23: PBS solvent control group (3 replicates).

T-20, T-20-6.21, T-20-6.23: 20 μmol/LHQ experimental group (3 repetitions).

A: Indicating that the sample qualified to meet the requirements of the construction of two libraries.