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. 2017 Sep 30;8(56):95880–95895. doi: 10.18632/oncotarget.21431

Figure 1. High methylation contributes to the downregulated of miR-29b in gastric cancer.

Figure 1

(A) Real-time RT-PCR was conducted to examine the miR-29b expression levels in 84 cases gastric cancer tissues and adjacent non-tumor tissues. (B) The gastric cancer patients with low expression of miR-29b showed shorter survival time when compared with those with high miR-29b expression. (C) Real-time RT-PCR was conducted to examine the miR-29b expression levels in human gastric cancer cell lines (AGS, BGC-823, SGC-7901, and MGC-803) compared with normal human gastric mucosa epithelial cell line GES-1. ** P<0.01 vs. GES-1. (D) Methylation-specific PCR was conducted to examine the methylation status of miR-29b in human gastric cancer cell lines (AGS, BGC-823, SGC-7901, and MGC-803) and normal human gastric mucosa epithelial cell line GES-1. (E) Gastric cancer cell lines were treated with DNA methyltransferase inhibitor 5-aza for 48 h, and real-time RT-PCR was conducted to examine the expression levels of miR-29b. Untreated gastric cancer cell lines were used as control group. ** P<0.01 vs. untreated. Data were expressed as the group means ± SD.