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. 2017 Sep 30;8(56):95880–95895. doi: 10.18632/oncotarget.21431

Figure 4. The expression of LASP1 was negatively mediated by miR-29b in gastric cancer cells.

Figure 4

AGS and BGC-823 cells were transfected with miR-29b mimic or miR-NC mimic, respectively. After transfection, (A) real-time PCR and (B) western blot were performed to determine the mRNA and protein expression of LASP1. For (A-B)** P<0.01 vs. miR-NC. After that, AGS and BGC-823 cells were transfected with miR-29b inhibitor or NC inhibitor, respectively. (C) After transfection, real-time PCR was conducted to examine the miR-29b expression. Then, (D) real-time PCR and (E) western blot were used to examine the mRNA and protein levels of LASP1. For (C-E) ** P<0.01 vs. NC inhibitor. Data were expressed as the group means ± SD.