Figure 5. EMT transition of cells cultured with PBS, TGFβ1, CAF-derived exosomes and exosomes with neutralizing TGFβ1 antibody in immunofluorescence.

(A) For SKOV-3 cells, expression of E-cadherin (upper) and Vimentin (lower) were changed during EMT, with F- actin and nuclear DAPI staining demonstrated in merge figures. (B) The expression of E-cadherin (upper) and Vimentin (lower) on CAOV-3 cells incubated with different treatments were detected by immunofluorescence staining. Similar variation of protein expressions were observed in both cell lines.