Figure 3. D561-0775 activated AMPK and its downstream signaling molecules.

(A & C) D561-0775 phosphorlated AMPK, ACC, and S6 on H1975 for 24 h and 48 h. (B & D) Statistical analysis of p-AMPK, AMPK, p-ACC, ACC, p-S6 after 24 h and 48 h. All data was presented as mean ± SD (n= 3, ^* p < 0.05, ^** p < 0.01). All western blot images were cropped from full-length blots.