Figure 2. Endothelial-supplied jagged-2 is not required to maintain HSPC function under homeostatic conditions.

(A–D) The total number of BMMNCs (A) and the WBC count (C) were quantified in adult Jag2^fl/fl or Jag2^ECKO mice. Lineage distribution of hematopoietic cells within the BM (B) and peripheral blood (D) was quantified in Jag2^fl/fl or Jag2^ECKO mice. For A and B, n = 3. For C, the number of dots indicates the number of biological replicates. For D, n = 9 for Jag2^fl/fl, n = 8 for Jag2^ECKO. (E) Representative flow cytometric gating of phenotypic Lin^–c-Kit⁺Sca1⁺CD150⁺CD48^– long-term HSCs (LT-HSCs). (F) Quantification of the number of phenotypic LT-HSCs per million BMMNCs in the femur of Jag2^fl/fl or Jag2^ECKO (n = 8 for each group). (G) Schematic view of competitive repopulating assay. (H) The percentage of CD45.2⁺ hematopoietic cells in the peripheral blood of CD45.1⁺ mice at 15.5 weeks after transplantation (n = 6 CD45.1 recipients for Jag2^fl/fl and n = 8 CD45.1 recipients for Jag2^ECKO mice). (I) Representative flow cytometric plots showing the multilineage engraftment. The CD45.2⁺ cells were further gated to reveal donor-derived CD3⁺ T cells and CD19⁺ B cells. (J) Quantification of multilineage engraftment within the CD45.2⁺ cells. Error bars indicate the SEM. *P < 0.05 and **P < 0.01, by unpaired 2-tailed t test. The numbers in the flow plots represent percentages of cells.