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. 2017 Nov 13;127(12):4498–4515. doi: 10.1172/JCI91553

Figure 12. Lnc-BM promotes recruitment of macrophages into the metastatic niche to form positive-feedback loop.

Figure 12

(A) RT-qPCR analysis of IL6ST-associated cytokine expression in BV2 cells incubated with conditioned media from indicated cells (n = 3 independent experiments, paired Student’s t test). (B and C) IHC detection of CD11b and Ly6G (B) or IF staining of IBA1 (C) in brain metastatic lesions of 231-Br cells harboring indicated shRNAs (related to Figure 2C; n = 5 animals per group, 3 sections per brain). B, brain tissue; Met, metastatic cancer cells. Scale bars: 200 μm (B), 100 μM (C). (D) ELISA detection of CCL2 concentration in conditioned media of 231-Br cells harboring indicated shRNAs treated with OSM (50 ng/ml) or IL-6 (50 ng/ml) for 12 hours (n = 3 independent experiments, paired Student’s t test). (E and F) RIP quantitative real-time PCR detection of Lnc-BM retrieved by indicated antibodies (E) or IB detection using indicated antibodies (F) in 231-Br cells treated with conditioned media from primed U937 cells in addition to indicated human antibodies (n = 3 independent experiments, paired Student’s t test; E). (G) Model of the action of Lnc-BM in mediating BCBM. Data are mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001.