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. 2017 Nov 13;127(12):4498–4515. doi: 10.1172/JCI91553

Figure 6. Lnc-BM impedes cancer cells from FasL-induced apoptosis.

Figure 6

(A and B) IF staining of cleaved caspase-3 in brain slices cocultured with 231-Br cells harboring indicated shRNAs (A) or in brain metastatic lesions (B, related to Figure 2C). Left panels: Representative images. Right panel: Statistical analysis (n = 3 independent experiments, unpaired Student’s t test). Scale bars: 100 μm. (C) FACS analyses of 231-Br cells transfected with indicated shRNAs followed by sFasL (200 ng/ml) treatment for 24 hours (n = 3 independent experiments, unpaired Student’s t test). (D and E) IF staining of cleaved caspase-3 in brain slices cocultured with 231-Br cells harboring scramble or Lnc-BM sgRNAs, followed by transfection of indicated expression constructs. (D) Representative images. (E) Statistical analysis (n = 3 independent experiments, unpaired Student’s t test). White arrows: apoptotic cells. Scale bars: 200 μm. Data are mean ± SEM; NS, P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001.