(A) BLI image (n = 7 and 8 animals) of mice 4 weeks after intracardiac injection of indicated cells. Left panel: Representative images. Right panel: Statistical analysis (1-way ANOVA). Scale bars: 200 μm. White arrows: mouse brain blood vessel. (B) Kaplan-Meier plot of brain-metastasis-free survival in the experiment of (A) (n = 7 and 8 animals, respectively, log rank test). (C) IHC staining of p-JAK2 in human breast cancer (n = 110 tissues) and adjacent normal tissues (NBT) (n = 28 tissues). Scale bars: 100 μm. (D) Kaplan-Meier RFS analysis of p-JAK2 in breast cancer patients detected by IHC (n = 42 and 43 tissues, respectively, log rank test). Data are mean ± SEM, ***P < 0.001.