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. 2017 Nov 6;127(12):4449–4461. doi: 10.1172/JCI96324

Figure 1. Human and mouse NASH are linked to induction of apoptosis and necroptosis in the liver.

Figure 1

(A) Immunoblots of total liver lysates from normal individuals and NASH patients. (B) qPCR analysis of gene expression in normal (n = 7) and NASH (n = 7) human livers. Data represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, 2-tailed unpaired Student’s t test. (C) H&E, sirius red, F4/80 immunofluorescence, and TUNEL staining of liver sections from male C57BL/6J mice fed chow or NASH diet. Scale bars: 100 μm. (D) Plasma ALT, AST, and HMGB1 levels in mice fed chow (n = 4) or NASH diet (n = 4). Data represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, 2-tailed unpaired Student’s t test. (E) qPCR analysis of hepatic gene expression. Data represent mean ± SEM. **P < 0.01; ***P < 0.001, 2-tailed unpaired Student’s t test. (F) Immunoblots of total liver lysates from mice fed chow or NASH diet. (G) qPCR analysis of Nrg4 expression in eWAT and BAT. Data represent mean ± SEM. *P < 0.05, 2-tailed unpaired Student’s t test.