Figure 10. Angpt1^p.R494* cannot replace WT Angpt1 in SC development.

Compared with littermate flox/flox and p.R494*/flox controls, at P21, Angpt1^{p.R494*/ΔE16.5} mice exhibited a hypomorphic SC with reduced PROX1 expression resembling that of Angpt1^WBΔE16.5 mice. This finding confirms that the Angpt1^p.R494* allele is functionally null in the context of SC development. n = 5 (flox/flox), 3 (p.R494*/flox and p.R494*/ΔE16.5), and 8 (WBΔE16.5) animals per group. **P < 0.01, ***P < 0.001 vs. flox/flox as determined by 1-way ANOVA followed by Bonferroni’s correction. AFU, background subtracted arbitrary fluorescence units. Scale bars: 50 μm; ×20 fields comprise an area of 65,536 μm².