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. 2013 Mar;15(2):220–226. doi: 10.1016/j.jmoldx.2012.10.002

Figure 1.

Figure 1

Detection of BRAF c.1798_1799delinsAA (p.V600K) by single-base primer extension MALDI-TOF MS. A two-primer strategy was used to detect the variant nucleotides in the first and second base positions of codon 600. A and C: Single peaks representing the WT nucleotides at the first and second positions of codon 600, G and T, respectively (black arrows). B and D: Heterozygous variant peaks (white arrows) representing an indel (GT>AA) involving positions 1 and 2 of BRAF codon 600, resulting in a p.V600K change.