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. 2013 Mar;15(2):270–279. doi: 10.1016/j.jmoldx.2012.11.002

Figure 1.

Figure 1

Analytical performance of the developed real-time PCR assay to measure the DNA methylation of the PITX2 gene locus. DNA samples with defined levels of DNA methylation were prepared by mixing artificially methylated DNA and DNA from sperm. Each mixture was analyzed in quadruplicate, and each measurement reflects the result from a single PCR.