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Establishment of a methylation cut-off for patient stratification into groups of patients at high risk and low risk of BCR. Leftover DNAs from 14 patient samples, which reflected the cut-off in a previous sample cohort,15 were measured using the developed PITX2 real-time PCR assay. The resulting cut-off was defined as the median of the 14 patient samples. Resulting cut-off: ΔΔCT = −3.96 (refers to 6.43% methylation).
