Figure 2.

PKCλ/ι deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, Rac1, and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. ^∗P < 0.05, ^∗∗P < 0.01.