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Detecting RNase P-specific target by PCR. Comparison of phenol-chloroform (A), spin column (B), and Automate Express (C) methods for purifying nucleic acids from blood samples spiked with different quantities of SPS and the use of this material in TaqMan assays for the detection of human RNase P. Samples were processed in triplicate. Data are expressed as means ± SD. IPC, internal positive control; SPS, sodium polyanetholsulfonate.
