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Detecting S. aureus-specific target by PCR. Comparison of phenol-chloroform (A), spin column (B), and Automate Express (C) methods for purifying nucleic acids from different volumes of blood culture fluid samples spiked with low-titer S. aureus and the use of this material in TaqMan assays for the detection of S. aureus. Each volume was tested in triplicate. Note that the S. aureus genome weighs approximately 3 fg.
