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. 2017 Nov 29;7:16585. doi: 10.1038/s41598-017-16828-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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STAT1 phosphorylation measures according to the NAbs titers in PBMC from MS patients. Expression of pSTAT1 determined by phosphoflow in monocytes, CD4+ and CD8+ T lymphocytes from MS patients after stimulation with 1000 IU/mL IFNβ1a (Avonex) in presence of several dilutions of a NAbs positive heterologous serum. As controls, non stimulated cells, IFNβ stimulated cells with B18R inhibitor and IFNβ stimulated cells with a NAbs negative heterologous serum, were included. The activation of pSTAT1 achieved after IFNβ stimulation decreased significantly in presence of the different titers of NAbs compared to the activation without serum in T cells and monocytes. In presence of NAbs negative serum no decrease were detected. Data are shown as percentage of cells expressing pSTAT1. Significant p values are shown with asterisk: *p ≤ 0.05. [NAb+]: positive neutralizing antibodies, [NAb−]: negative neutralizing antibodies.