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. 2017 Nov 29;7:16547. doi: 10.1038/s41598-017-16702-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Construction and validation of Lenti-IL-27HA and Lenti-IL-35HA. (A,B). Graphic representation of the lentiviral constructs used to express IL-27 (A) and IL-35 (B). (C,D) Cytokines production was assessed by ELISA (IL-27, C) or WB (IL-35, D). (E–G). Affinity purified IL-27 (white bars) and IL-35 (black bars) were used to treat polarized CD4⁺ T cells and measure mRNA expression of IL-2 (E), IL-17 (F), gm-csf (G), and IL-10 (F) (mean ± sd). NC = no cytokines. *p < 0.05, **p < 0.001, ***p < 0.0001 (1 way Anova). Blot in panel (D) has been cropped, and full blot is presented in Supplementary Fig. 7. We show here one representative out of three independent experiments.