Figure 6.

IL-27 and IL-35 gene therapy modulate gene expression in resident microglia and in infiltrating T and myeloid cells. We sorted CNS-resident and -infiltrating cells from gene therapy-treated EAE mice dividing them in T cells (A), microglia (B), experienced (C), and newly infiltrating monocytes/moDCs (D), according to the strategy depicted in see Supplementary Fig. 4. Gene expression for gm-csf, tbet, foxp3, rorc (A), arg1, ym1, inos, and pd-l1 (B–D) was measured by real time RT-PCR and expressed as arbitrary units AU (mean ± sd). *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired t-test, n = 6 mice/group).