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. 2017 Nov 29;7:16564. doi: 10.1038/s41598-017-16709-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of papaya pectin in HCT116, HT29 and PC3 apoptosis by flow cytometry. Cancer cells had induced late apoptosis/necroptosis with 3PP. Cells were treated with 0.20% of 3 PP and 4 PP pectin for 24 h. (A) Percentage of viable cells. (B) Percentage of apoptotic cells. (C) Percentage of necroptotic cells. (D) Percentage of necrotic cells. (E) Flow cytometry plots of HCT116. (F) Flow cytometry plots of HT29. (G) Flow cytometry plots of PC3. The results were expressed in percentage of cells in comparison with control (no treatment). Results were represented as mean ± SD of two independent WSF samples, each one performed in technical triplicate, from the biological duplicate (n = 4). *P < 0.05 vs control, according to Dunnett’s test. PP: papaya pectin (water-soluble fraction).