Fig. 1.

Myosin VI is distributed throughout the nucleus. a Cartoon depiction of the MVI domains and key features discussed in the text. b Immunofluorescence staining against MVI (magenta) and DNA (cyan) in HeLa cells and isolated nuclei (see Supplementary Fig. 1 for nuclei images). Arrows highlight filamentous structures within the nucleus. Images were acquired at the mid-point of the nucleus. Scale bar 10 μm for whole images and 1 μm for inserts. c Western blot against MVI following HeLa cell fractionation. Tubulin and lamin B are used as cytoplasmic and nuclear markers, respectively. d Representative images of transiently expressed NI- and LI-GFP-MVI in HeLa cells combined with DNA staining (cyan) (Supplementary Fig. 3). Images acquired as in B. e Pull-down of recombinant MVI using a 500 bp DNA substrate. f Plot of band intensities normalised to the input sample (5 μM). Errors bars represent SEM from three independent preparations. (**p < 0.001 by two-tailed t-test between presence and absence of DNA)