Fig. 5.
NDP52-dependent dimerisation of myosin VI. a FRET titration of FITC-MVITAIL against 1 μM AF555-MVITAIL ± DNA (20 μM) and NDP52 (20 μM). Data fitting was performed as described in Methods giving a K d as plotted in b. See Supplementary Fig. 7a–c for raw intensity data. b Plot of K d from titrations in a and Supplementary Fig. 7d–g (error bars represent SEM from three independent experiments). c Velocity histogram from sliding filament assay with MVI immobilised alone (blue), through antibody (red) and NDP52 (green). Insert shows first frame (red) and after 60 s (green). d Two routes of NDP52-dependent dimerisation with different stoichiometry. (i) NDP52 unfolds MVI then directly recruits a second molecule. (ii) Each MVI is unfolded by an individual NDP52