Fig. 8.

Myosin VI is linked to oestrogen signalling. a Pull-down of 2 μM CBD by 10 μM GST-ER. P and S represent pellet and supernatant fractions. b Expression of ER gene targets following siRNA knockdown of MVI in MCF-7 cells. Expression is plotted as a percentage of expression in mock cells. ER (ESR1) and β-actin were used to reflect global changes in transcription. c Luciferase reporter assay driven by the ERE promoter in MCF-7 cells. Estradiol led to a 5-fold increase in promoter activity. siRNA knockdown of MVI led to a 3-fold reduction in activity. Ethanol was used as a carrier control for the experiments (error bars represent SEM from three independent experiments **p < 0.001 by two-tailed t-test). d Working model of MVI recruitment by the ER. MVI binds to the ER and then gets activated by NDP52 (or CoCoA), which enables binding to RNAPII through actin. Association with NDP52, or CoCoA, ties MVI with the general transcription co-activators to initiate recruitment of RNAPII