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. 2017 Nov 29;7:16556. doi: 10.1038/s41598-017-16448-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Levels of mitochondrial and nuclear DNA in micro- and nano- vesicles from ID2-treated placentae. Total DNA was extracted from micro- and nano- vesicles from first trimester human placentae that were cultured in the absence of antibodies (UT), or in the presence of isotype-matched control IgG (IgG) or ID2 antibodies (50 µg/mL, n = 6 placentae). Quantitative digital PCR using probes against ND1 and RNase P was performed. The absolute copy number of mtDNA (A) and nuclear DNA (B) in one nanogram of total DNA is depicted. As data was normally distributed, a repeated measures one-way ANOVA was performed between treatment groups to test for statistical differences (*p < 0.05, **p < 0.01).