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. 2017 Nov 29;7:16542. doi: 10.1038/s41598-017-16319-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of HaAce RNAi and HaFpps4 RNAi on the larvae. At 1d fourth-instar, larvae were microinjected with dsRNA of HaFpps4 (dsFpps4), dsRNA of HaAce (dsAce) or dsRNA of Green fluorescent protein (dsGFP) (control). The expression levels of the mevalonate pathway genes (A,D), the changes of JH titer (B,E) and the larvae development (C,F) were analyzed. The abnormal molting observed in dsFpps4 injection was labeled with red arrow. The standard error is represented by the error bar. Asterisks indicate statistically significant differences between groups (t-test): *P < 0.05, **P < 0.01, ns: no significant difference.