Table 1.
Primers used in this experiment.
| Primer | Forward primer (5′-3′) | Reverse primer (5′-3′) | Size |
|---|---|---|---|
| RT-PCR analysis | |||
| Actin | CCGTCCACAATGAAGATCAA | ATCGACAATGTTCCGCATTC | 330 |
| Ace | TGGCAACTGTGTTCCAAAAG | GAGAGCATGGCACAAGTGAA | 407 |
| Hmgr | GAGCACCGGCTATTAAGCTG | CAGAGTTTTGGCATCGGTTT | 416 |
| Hmgs | ATGGCAGAAAGGCGATAGTG | TCTGGTGATGCATTGAGGAA | 406 |
| Mk | TTACGGGCAAATTCTCTTGG | TGATGGAGATCCGTGCATTA | 419 |
| Pk | GTTGGGTGACGGAGAGTACA | TCCACCTCCTGTTTTCCTTCA | 351 |
| Ippi | CGGTGAGAGTGGAAAAGAGG | CAACTCTGGGTCCATCTGGT | 413 |
| Dpp | TGACTCTACACACTGGCCTG | TTGGTCCGGCATCAAATGTG | 365 |
| Fpps1 | GTCTGATGGAAGTCCTGCAAAT | GGTCTCTGCTGCCGTAGTATTC | 474 |
| Fpps2 | CCAGAGAACATAACGGAGGAGA | AGAGATATGGGCAGCTTGTACG | 416 |
| Fpps3 | AATGGGACAACACTTGGACTTC | GGTAGCCTCAAATCACCGTAAA | 426 |
| Fpps4 | CGAAGCCATCACGAAGTACA | CCTCTTGTGACTTGCTGGAT | 426 |
| qRT-PCR analysis | |||
| Actin | TGGTATTGCTGACCGTATGC | CTGTTGGAAGGTGGAGAGG | 142 |
| Ace | TCCAAAAGGAGAATGGAACG | TCCCCATCAGCATATCCAAT | 145 |
| Hmgr | ATCGTGGCCACATTAGCTCT | CATCAGCGGCTTGGTACTCT | 133 |
| Hmgs | AAGGCAGAGTGTGGAGCCTA | GCCGGGGAACAGTATGTCTA | 112 |
| Mk | ATGCCCTGCAGAATAACCAG | CAGCTCCAATAGTTAACTCA | 132 |
| Pk | TCTGGAGTTGACGATGTTGC | AACCAGACAGCAATCCCAAT | 133 |
| Ippi | CGCTGAAACCACAGACTGAA | TTCCCACGAAGTTGTCCTTC | 108 |
| Dpp | TGTATACGGTGGCTTCGTCA | GCATTTCAGGCCAGTGTGTA | 101 |
| Fpps1 | CTGCACACAGCACGATTTCT | TAGTCGCCGAAGTACCGACT | 145 |
| Fpps2 | AAGGATTGCCAATGTTACGG | ATGGAACTCCACCCTTTCCT | 109 |
| Fpps3 | CAGGAAGGTGCTTGAACACA | CTGGCCAGCTTGAGTTTCTC | 123 |
| Fpps4 | GAGACTGGCAAGCACATTGA | CAGCATTTTGTAAGCCAGCA | 123 |
| RNAi analysis | |||
| Ace | TAATACGACTCACTATAGGGTGGCAACTGTGTTCCAAAAG | TAATACGACTCACTATAGGGAGAGCATGGCACAAGTGAA | 406 |
| Fpps4 | TAATACGACTCACTATAGGGACATCGTAGAAGGCACAGAGA | TAATACGACTCACTATAGGGTCTGTAAGGCGACAACTG | 519 |
| GFP | TAATACGACTCACTATAGGGGCAACATACGGAAAACTTACC | TAATACGACTCACTATAGGGTGTGTGGACAGGTAATGGTTG | |