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. 2017 Nov 29;7:16575. doi: 10.1038/s41598-017-16857-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of LC-MS/MS quantification of key metabolite changes in plasma samples of animals undergoing asphyxial and dysrhythmic CA. Results, expressed in µmol/L, are graphed as box plots, indicating median values and upper and lower quartile distributions, at baseline, CA (last minute), CPR (last minute), ROSC and post-ROSC (1, 2, 3, 4, 24 h). (a) TCA cycle, urea cycle, lipid metabolism intermediates. (b) Anaplerotic replenishing of TCA cycle. Asterisks indicate statistical significance regarding the within group comparison of each phase with the previous one calculated by paired Wilcoxon t-test (*p-value ≤ 0.05, **p-value ≤ 0.01, ***p-value ≤ 0.001).