Fig. 2. TMB construction and activation. (a) Absorption spectra; (b) photoluminescence spectra; (c) photographs under UV excitation; (d) agarose gel electrophoresis of CdTe/CdS/ZnS QDs, mixture of QDs and MB (without TCEP reduction), and TMB. (e) Activation of TMB with perfect-matched DNA1 and single-base-mismatched DNA2 measured by photoluminescence spectra. (f) Digestion of TMB and MB with DNase I measured by the PL intensities of the probes. DNA1-activated TMB and MB are used as controls.