Table 1. Summary of spectral overlap J, Förster distance R₀, and FRET efficiency E of each FRET pair of TMB. See Experimental section for detailed calculations (ESI†).

FRET pair	J (cm3 M–1)	R 0 (nm)	r (close) (nm)	r (open) (nm)	E (close)	E (open)
QD–BHQ3 (n = 10)	5.04 × 10–14	4.0	4.0	14.0	91%	—
QD–Ce6 (n = 10)	3.44 × 10–13	5.5	6.4	6.4	—	80%
Ce6–BHQ3 (n = 1)	6.94 × 10–14	3.4	2.0	7.6	96%	0.79%