Fig. 2. The T _C of dithioether PCs determined by steady-state anisotropic measurements. (a) Anisotropic curve of C18:0 dithioether PC 4, a sudden change of slope indicates the membrane transitions from a gel state to a liquid state at 59 °C. (b) The T _C of C12:0, C14:0, C16:0 and C18:0 dithioether PC 1–4 are 7 °C, 28 °C, 47 °C and 59 °C, respectively, slightly higher than –2 °C, 24 °C, 41 °C and 55 °C of their natural PC analogs. (c) DPH anisotropy of dithioether PC 1–5 and their natural analogs at 23 °C. Error bars denote standard deviation (n = 3).