Fig. 4. Time-lapse fluorescence microscopy images of shrinking vesicles. (a) The GFP leaked from DOPC/5 GUV upon the shrinkage of the vesicle by PLA2 treatment. The GUV membrane was stained with Texas Red DHPE. (b) The 100 nm red fluorescent sulfate-modified polystyrene nanoparticles were contained and concentrated in DOPC/5 GUV as the vesicle shrank. The membrane was stained with NBD-PE.
