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. 2017 Oct 25;9(11):311. doi: 10.3390/v9110311

Figure 5.

Figure 5

Southern blot assay of DNA extracts of: 1, CpCDV-infected N. benthamiana; 2, healthy N. benthamiana; 3, CpCDV-infected watermelon plant 6/14 (leaf extract); 4, CpCDV-infected watermelon plant 6/23 (leaf extract); 5, CpCDV-infected watermelon plant 4/27 (fruit extract); 6, healthy watermelon (leaf plus fruit); P, 6 ng of CpCDV genome full length fragment; M, 1 Kb Ladder Plus DNA ladder: the pale 1650 bp band is indicated (Thermo Fisher Scientific, Waltham, MA, USA). On the right, the four CpCDV genome forms found in watermelon leaf and fruit extracts are shown by black arrows; the forms of the double-stranded DNA are indicated: open circular (OC), linear (Lin) and covalently closed circular (CCC), single-stranded DNA is marked (ssDNA) (a) ethidium bromide-stained agarose gel showing the high molecular weight genomic DNA bands (white arrow); (b) autoradiography film resulting from chemiluminescent detection using CpCDV DIG-labelled probe.