Table 1.
Data collection, image pre-processing, and PHENIX refinement.
| Parameter | LuIII |
|---|---|
| Total No. of micrographs | 722 |
| Defocus range (μm) | 0.04–4.39 |
| Electron dose (e−/Å2) | 75 |
| No. of frames/micrograph | 50 |
| Pixel size (Å/pixel) | 1.064 |
| Starting No. of particles | 20,142 |
| No. of particles used for final map | 18,134 |
| Inverse B-factor used for final map (Å2) | 50 |
| Resolution of final map (Å) | 3.17 |
| Residue range | 37–587 |
| Map CC | 0.88 |
| RMSD (Å) | |
| Bonds | 0.0 |
| Angles | 0.8 |
| All-atom clash score | 11.8 |
| Ramachandran Plot (%) | |
| Favored | 97.8 |
| Allowed | 2.0 |
| Outliers | 0.2 |
| Rotamer outliers (%) | 0.2 |
| No. of Cβ deviations | 0.0 |