Figure 4.

Construction of an eGFP-tagged PVY intron-containing infectious cDNA clone: (a) Schematic representation of the eGFP insertion; (b) Symptoms and eGFP expression of PVY-intron-GFP and PVY-intron infected N. tabacum plants. The upper panels show symptomatic plants photographed at 15 days post infiltration and the lower panels show eGFP expression in systemically infected leaf tissues observed by fluorescence microscope; (c) Total proteins extracted from systemically infected plants or un-infiltrated control plants were analyzed by Western blotting using an antibody against the PVY coat protein (CP). The Coomassie blue-stained Rubisco large subunit (Rub L) is showed as a loading control.