Figure 2.

Real-time cell analysis of HEp-2 and Rat-2 cells infected with CPXV crust and passages reveals no differences among passages but heterogeneities of the crust. (a) HEp-2 and (b) Rat-2 cells were seeded in 96-well E-plates and cell adherence was measured over time. About 24 h after seeding, cells were infected with purified CPXV originating from a rat crust or from different passages (P1–P5) at an MOI of 0.1 (arrow). Shown are mean values ± SD of biological triplicates of passages measured in technical quadruplicates (n = 12) or six technical replicates of the crust, which cluster in two different courses (crust_I and II) with each n = 3. NTC: non-infected cells.