Figure 4.

Viral proteins changing in an adaptation-associated manner during passaging in HEp-2 and Rat 2 cells. Proteins are sorted according to adaptation-associated time courses with distances ≤0.1 to reference profiles A–D, which are shown in Figure S1. (a) A single viral protein (G13) displays comparable courses during passaging in both cell lines (cell line unspecific). Proteins changing in a cell-line-independent manner were identified in (b) Rat-2- and (c) HEp-2-passaged virions. Shown are normalized LFQ intensities (log₂) of ANOVA-significant proteins. Error bars indicate means ± SD of three biological replicates (passages 1–5) or three technical replicates (passage 0: crust).