Table 1.
Mutations in coding sequences during CPXV crust passaging.
| CDS a | Pos. b | Nucleotide Change | PE c | Amino Acid Change | Variant Frequency HEp-2 | Variant Frequency Rat-2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P0 | P1 | P2 | P3 | P4 | P5 | P1 | P2 | P3 | P4 | P5 | |||||
| A12R | 728 | A→G | S | K→R | 1.2 | ||||||||||
| A24R | 1149 | (A)8→(A)9 | FS | none | 1.4 | ||||||||||
| A25R | 269 | A→C | S | K→T | 3.1 | 2.7 | 4.2 | 6.4 | 2.9 | 11.4 | 2.2 | 4.2 | 2.7 | 9.5 | 4.6 |
| A25R | 4 | (A)8→(A)9 | FS | 1.3 | 1.4 | ||||||||||
| A26L | 1091 | G→T | S | H→Q | 1.1 | ||||||||||
| A26L | 1118 | (GTT)8→(GTT)9 | I | P→QP | 3.1 | 2.7 | 2.9 | 2.6 | 3.4 | 2.6 | 4.2 | 2.5 | |||
| A26L | 1115 | (GTT)8→(GTT)7 | D | QP→P | 4.6 | 4.3 | 3.8 | 3.8 | 4.2 | 4.6 | |||||
| A26L | 1093 | (TTG)8→(TTG)7 | D | Q→ | 3.7 | 5.0 | 3.4 | ||||||||
| A26L | 1093 | (TTG)8→(TTG)9 | I | →Q | 4.0 | 2.6 | 2.8 | ||||||||
| A9R | 259 | (A)8→(A)9 | FS | 1.5 | |||||||||||
| C1L | 465 | C→A | S | R→S | 1.2 | ||||||||||
| C2L | 168 | C→T | N | none | 1.5 | ||||||||||
| D6L | 613 | ATA→TCC | S | Y→G | 1.9 | ||||||||||
| D6L | 615 | A→T | T | 1.0 | 1.1 | ||||||||||
| D6L | 616 | +TCC | I | →G | 8.8 | 7.4 | |||||||||
| D6L | 611 | C→T | S | S→N | 8.7 | 7.4 | |||||||||
| D6L | 606 | G→A | N | none | 8.5 | 7.1 | |||||||||
| E10R | 474 | (T)7→(T)8 | FS | 2.9 | 2.0 | 1.7 | 2.2 | ||||||||
| E10R | 481 | (T)7→(T)8 | FS | 2.6 | |||||||||||
| E10R | 716 | A→T | S | E→V | 1.8 | ||||||||||
| H3R | 28 | A→G | S | N→D | 1.3 | ||||||||||
| J4L | 101 | +GG | FS | 1.1 | |||||||||||
| J6R | 319 | A→G | S | K→E | 2.1 | ||||||||||
| L6L | 1084 | (T)10→(T)11 | FS | 1.9 | |||||||||||
| O4R | 1648 | T→C | S | S→P | 1.1 | ||||||||||
Variant frequencies are shown in %; P0: crust; a Coding sequence (CDS; gene name according to CPXV GRI-90); b Position (Pos.) in gene; c Protein effect, S: Substitution, FS: Frame Shift, I: Insertion, D: Deletion, T: Truncation, N: None.