Figure 4.

PAXX does not affect the proportion of endless HSV-1 genomes, but promotes genome replication. (A) Linear structure of the HSV-1 genome, indicating repetitive elements (a, a′, a″) and the S, K, and Q fragments created by cleavage with BamHI (selected restriction sites are denoted by red lines); (B) WT and PAXX^−/− cells were infected at MOI 4, and total cellular and viral DNA was isolated. The HSV-1 Q, K, and S fragments were visualised by southern blot following digestion with BamHI; (C) WT and PAXX^−/− RPE-1 cells were infected at an MOI of 4, and genome copy numbers were quantified using TaqMan qPCR. n = 3. Statistical significance, calculated by Student’s t-test, is denoted by stars—** = p < 0.02, *** = p < 0.01. n = 3. Error bars denote mean +/− SEM.