Figure 3.

Enhanced growth and differentiation of myoblasts on graphene nanomaterial-coated substrates. (a) Characterizations of GO- and rGO-coated glass substrates by atomic force microscopy (AFM). (b) Myogenic differentiation on uncoated, GO- and rGO-coated glass substrates. C2C12 cells were grown in growth media (Dulbecco’s modified Eagle’s medium, DMEM, containing 10% fetal bovine serum and 1% antibiotic-antimyotic solution) for 1 day and then incubated in differentiation media (DMEM containing 2% horse serum and 1% antibiotic-antimyotic solution) for 5 days. Reproduced with permission from [32]. Copyright Elsevier Ltd, 2012.