. 2017 Oct 31;56(47):6257–6267. doi: 10.1021/acs.biochem.7b00556

Table 1. Buffer Dependence of Nitrocefin Hydrolysis by BlaC^a.

buffer	k_cat (s^–1)	K_m (μM)	k_cat/K_m (× 10⁵ M^–1 s^–1)
NaPi pH 6.0	107 ± 6	147 ± 14	7.3 ± 0.4
NaPi pH 7.0	64 ± 6	153 ± 23	4.2 ± 0.2
MES pH 6.0	69 ± 1	38 ± 7	18 ± 3
BIS-TRIS pH 6.0	83 ± 3	61 ± 7	14 ± 1

Buffer concentrations were each 100 mM. Errors represent the standard deviation over triplicate measurements.

Table 1. Buffer Dependence of Nitrocefin Hydrolysis by BlaCa.