Table 3. Rates of BlaC Activity Recovery from Clavulanic Acid Inhibition.
| buffera | [BlaC] (μM) | [clavulanic acid] (μM) | ratio | 50% recovery time (h) | turnover rate (10–4 s–1) |
|---|---|---|---|---|---|
| MESb | 20 | 100 | 1:5 | 14 ± 1 | 1.03 ± 0.07 |
| MESc | 100 | 100 | 1:1 | 1.8 ± 0.8 | 1.5 ± 0.7 |
| MESc | 100 | 300 | 1:3 | 7 ± 0.8 | 1.2 ± 0.1 |
| MESc | 100 | 500 | 1:5 | 13.8 ± 0.8 | 1.01 ± 0.06 |
| MESc | 100 | 1000 | 1:10 | 28 ± 0.8 | 0.99 ± 0.03 |
| MESc | 100 | 1500 | 1:15 | 43.5 ± 1 | 0.96 ± 0.02 |
| MESd | 300 | 1500 | 1:5 | 6.0 ± 0.6 | 2.3 ± 0.2 |
| MES + 100 mM acetic acidc | 100 | 500 | 1:5 | 36 ± 3 | 0.38 ± 0.03 |
| MES + 100 mM Na2SO4c | 100 | 500 | 1:5 | 2.0 ± 0.5 | 7 ± 2 |
| NaPib | 20 | 100 | 1:5 | 0.63 ± 0.06 | 22 ± 2 |
| NaPid | 300 | 1500 | 1:5 | 0.82 ± 0.01 | 16.8 ± 0.3 |
a
Buffers were all 100 mM, pH 6.4.
b
Errors are the standard deviations over four replicates.
c
Errors are the estimated error in half-time determination of single experiments.
d
Errors are the standard deviations over two replicates.