Skip to main content
. 2017 Sep 12;11(11):10689–10703. doi: 10.1021/acsnano.7b04743

Figure 5.

Figure 5

Detection of endolysosomal lipid accumulation in live cells. (a) Schematics of the ss(GT)6-(8,6) nanotube complexes in macrophages treated with U18666A or Lalistat 3a2. (b) Overlay of transmitted light with hyperspectral image of RAW 264.7 macrophages incubated with ss(GT)6-(8,6) complex under the specified treatments. Color legend maps to nanotube emission peak wavelength. Scale bar = 50 μm. (c) Histogram of emission wavelengths of all pixels from the hyperspectral images, with a bin size of 1 nm. (d) Schematic of the optical setup for high-throughput measurements of ss(GT)6-(8,6) emission in live cells. (e) Spectra of ss(GT)6-(8,6) emission from live RAW 264.7 cells incubated in normal media (control) and in media with U18666A. (f) Mean center wavelengths of ss(GT)6-(8,6) emission from n = 5 technical replicates, acquired using hyperspectral imaging (I) and spectroscopy (S). Mean values were compared using a one-way ANOVA with Sidak’s multiple comparison test. Error bars are standard error of the mean. *** = p < 0.001, **** = p < 0.0001.