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. 2017 Oct 28;9(11):1184. doi: 10.3390/nu9111184

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of skullcap extract (ethanol, hexane, chloroform, ethyl acetate, butanol, and water) on ex vivo splenocytes from ovalbumin (OVA)-sensitized BALB/c mice. (A) Interleukin (IL)-4 and (B) Interferon (IFN)-γ were quantified by enzyme-linked immunosorbent assay (ELISA) using splenocytes; (C) Cell viability and cytotoxicity were measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; (D) Inhibition rate (%). Values are presented as mean ± standard deviation (SD) (n = 4 in each group). Data were analyzed by analysis of variance (ANOVA), followed by Duncan’s multiple-range test. Data are statistically different (p < 0.05) among those columns with different symbols. The order of value is termed as order of alphabet.