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Effects of skullcap hexane fraction (50 µg/L on OVA-induced Th2 immune response ex vivo. (A) Th2-associated cytokines such as IL-4, IL-5, IL-10 and IL-13; (B) Th1-associated cytokines, such as IFN-γ and IL-12, were quantified by enzyme-linked immunosorbent assay (ELISA) using splenocytes. Values are presented as mean ± SD (n = 4 in each group). Data were analyzed by ANOVA, followed by Duncan’s multiple-range test. * p < 0.05, ** p < 0.01, significantly different from the OVA value.
