Effects of skullcap hexane fraction and key compound (linoleic acid) on TEER in Caco-2 cell monolayers and ovalbumin (OVA) flux in Caco-2 cell monolayers. (A,B) Effects of skullcap hexane fraction (100 µg/mL) on TEER value and OVA influx. (C,D) Effects of linoleic acid, a key compound in the hexane fraction, on TEER and OVA flux. Values are presented as mean ± SD (n = 4 in each group). Data were analyzed by ANOVA, followed by Duncan’s multiple-range test. (A,B) * p < 0.05, ** p < 0.01, significantly different from the control value. (C,D) Data are statistically different (p < 0.05) among those columns with different symbols. The order of value is designated as alphabetical order.