Figure 5.

Inhibition of t-BHP-induced apoptotic cell death by barley sprout extract (BSE) in HepG2 cells. Cells were pretreated with BSE (1 mg/mL) for 12 h before treatment with t-BHP (300 µM) for 18 h, followed by (A) FACS analysis of propidium iodide uptake and annexin V binding in non-permeabilized cells (lower-left, live cells; lower-right, early apoptotic cells; upper-right, late apoptotic cells); (B) quantification of apoptotic and (C) live cells based on 3 independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple comparisons test (p < 0.05).