Figure 1.

Flow cytometry gating strategy. Granulocyte and monocyte populations gated based on their forward (FSC) vs. side scatter (SSC) properties (A). Granulocyte populations were confirmed to be CD16⁺ with granulocyte-platelet aggregates identified by quadrant gating as CD16⁺ and CD42a⁺ cells (B) and relative expression of αM integrin expression on granulocytes defined as CD11b⁺ (blue) compared to unstained control (red) via histogram (C). Monocyte populations were confirmed to be CD14⁺. Monocytes sub-populations are defined by gating of CD14⁺⁺CD16⁻ (classical), CD14⁺⁺CD16⁺ (intermediate) and CD14⁺CD16⁺ (non-classical) (D). Monocyte-platelet aggregates were identified by quadrant gating as CD14⁺ and CD42a⁺ cells (E). Relative expression of αM integrin expression on monocytes defined as CD11b⁺ (blue) compared to unstained control (red) via histogram (F). Platelet population was identified using histogram analysis of cells CD42a⁺ (G) with P-selectin expression on platelets determined through CD62P histogram analysis of CD42a⁺ population (H).